《植物生理学报》 2017, 53(10): 1877-1884

干旱胁迫和复水处理对玉米叶片光合电子传递特性的影响

刘佳, 杨永红, 郝引川, 张兴华, 薛吉全^*, 张仁和^*

西北农林科技大学农学院/农业部西北旱区玉米生物学与遗传育种国家重点实验室, 陕西杨凌712100

通信作者：薛吉全；E-mail: xjq2934@163.com; zhangrenhe1975@163.com

摘　要：

本文以2个玉米品种‘陕单609’ (抗旱性强)和‘陕单902’ (抗旱性弱)为材料, 采用盆栽控水方式, 通过叶绿素荧光诱导动力学曲线和820 nm光反射曲线测定分析了干旱胁迫10 d及复水10 d对2个玉米品种光系统电子传递特性的影响。结果显示, 随着干旱胁迫程度的加剧, ‘陕单609’和‘陕单902’的叶片光系统II (PSII)、光系统I (PSI)以及两个光系统间电子传递的活性均下降, 但‘陕单609’光合电子传递参数下降幅度较‘陕单902’小。复水后‘陕单902’从PSII供体侧到PSI末端受体侧的光合电子传递活性恢复缓慢, 而‘陕单609’的能够快速恢复到对照水平。这些结果表明, 与‘陕单902’相比, ‘陕单609’在干旱胁迫和复水处理下能较好地协调PSII和PSI电子传递变化, 这种自我保护机制维持了光合系统的稳定性, 是其适应干旱环境的重要生理原因。

关键词：玉米叶片; 干旱胁迫和复水处理; 光合电子传递; OJIP荧光分析

收稿：2017-05-27   修定：2017-09-25

资助：国家重点研发计划项目(2017YFD0300304)、陕西省农业科技创新转化项目(NYKJ-2015-16)和杨凌农业科技计划项目(2015NY-03)。

Effects of photosynthetic electron transport chain of maize leaves under drought stress and re-watering

LIU Jia, YANG Yong-Hong, HAO Yin-Chuan, ZHANG Xing-Hua, XUE Ji-Quan^*, ZHANG Ren-He^*

College of Agronomy, Northwest A&F University / Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, Ministry of Agriculture, Yangling, Shaanxi 712100, China

Corresponding author: XUE Ji-Quan; E-mail: xjq2934@163.com; zhangrenhe1975@163.com

Abstract:

Two maize cultivars including drought-tolerant ‘shaandan 609’ and drought-sensitive ‘shaandan 902’ were subjected to 10-day drought stress and subsequently re-watered for 10-day in pot experiment. In the study, simultaneously measurements of prompt chlorophyll a fluorescence and modulated 820 nm reflection were employed to investigate the effects of drought and re-watering on photosynthetic electron transport chain in maize leaves. The results showed that the activity of photosystem II (PSII), electron transfer between PSII and PSI, and photosystem I (PSI) were decreased in both maize cultivars with drought stress, while the reductions of photosynthetic electron transport parameters occurred earlier and were more apparent in ‘shaandan 902’ than in ‘shaandan 609’. After re-watering, the electron transfer from the PSII donor side to the PSI end receptor of ‘shaandan 609’ was rapidly restored to the control level, while ‘shaandan 902’ was gradual recovery. Our results revealed that the ‘shaandan 609’ possessed an advantage over ‘shaandan 902’ in close coordination between PSII and PSI under drought stress and re-watering. Such protection mechanism contributed to ensuring the stability of photosystems, which appeared to be a critical physiological mechanism allowing ‘shaandan 609’ to withstand drought environment.

Key words: maize leaves; drought stress and re-watering; photosynthetic electron transport; JIP-test